Analysis of cytokine production by peanut-reactive T cells identifies residual Th2 effectors in highly allergic children who received peanut oral immunotherapy.

ABSTRACT:

Background

Only limited evidence is available regarding the cytokine repertoire of effector T cells associated with peanut allergy, and how these responses relate to IgE antibodies to peanut components.

Objective

To interrogate T cell effector cytokine populations induced by Ara h 1 and Ara h 2 among peanut allergic (PA) children in the context of IgE and to evaluate their modulation during oral immunotherapy (OIT).

Methods

Peanut-reactive effector T cells were analysed in conjunction with specific IgE profiles in PA children using intracellular staining and multiplex assay. Cytokine-expressing T cell subpopulations were visualized using SPICE.

Results

Ara h 2 dominated the antibody response to peanut as judged by prevalence and quantity among a cohort of child

SUBMITTER: Wisniewski JA 

PROVIDER: S-EPMC4472497 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature