A Phytase-Based Reporter System for Identification of Functional Secretion Signals in Bifidobacteria.

ABSTRACT: Health-promoting effects have been attributed to a number of Bifidobacterium sp. strains. These effects as well as the ability to colonise the host depend on secreted proteins. Moreover, rational design of protein secretion systems bears the potential for the generation of novel probiotic bifidobacteria with improved health-promoting or therapeutic properties. To date, there is only very limited data on secretion signals of bifidobacteria available. Using in silico analysis, we demonstrate that all bifidobacteria encode the major components of Sec-dependent secretion machineries but only B. longum strains harbour Tat protein translocation systems. A reporter plasmid for secretion signals in bifidobacteria was established by fusing the coding sequence of the signal peptide of a sialidase of

SUBMITTER: Osswald A 

PROVIDER: S-EPMC4472781 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature