Colorimetric quantification of sucrose in presence of thermo-sensitive polymers present in aqueous two-phase systems.

ABSTRACT: The use of biodegradable material such as simple carbohydrates and recyclable material such as thermo-sensitive polymers is in need to develop a sustainable aqueous two-phase system (ATPS) for the purification of biomolecules. Accurate determination of sucrose concentration is important in liquid-liquid equilibrium (LLE) study of carbohydrate-based ATPS. The well-established phenol-sulfuric acid method has been widely employed in the measurement of carbohydrate concentration. However, the presence of thermo-sensitive polymers, which has a lower critical solution temperature (LCST) below room temperature, in carbohydrate samples could hamper the precision of spectrophotometric analysis due to the formation of two phases or cloudiness in the sample. Thus, the following modifications were mad

SUBMITTER: Ramalakshmi S 

PROVIDER: S-EPMC4472962 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature