Regulation of Latent Membrane Protein 1 Signaling through Interaction with Cytoskeletal Proteins.

ABSTRACT:

Unlabelled

Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) induces constitutive signaling in EBV-infected cells to ensure the survival of the latently infected cells. LMP1 is localized to lipid raft domains to induce signaling. In the present study, a genome-wide screen based on bimolecular fluorescence complementation (BiFC) was performed to identify LMP1-binding proteins. Several actin cytoskeleton-associated proteins were identified in the screen. Overexpression of these proteins affected LMP1-induced signaling. BiFC between the identified proteins and LMP1 was localized to lipid raft domains and was dependent on LMP1-induced signaling. Proximity biotinylation assays with LMP1 induced biotinylation of the actin-associated proteins, which were shifted in molecular ma

SUBMITTER: Holthusen K 

PROVIDER: S-EPMC4473556 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature