SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy.

ABSTRACT: Single molecule localization based super-resolution fluorescence microscopy offers significantly higher spatial resolution than predicted by Abbe's resolution limit for far field optical microscopy. Such super-resolution images are reconstructed from wide-field or total internal reflection single molecule fluorescence recordings. Discrimination between emission of single fluorescent molecules and background noise fluctuations remains a great challenge in current data analysis. Here we present a real-time, and robust single molecule identification and localization algorithm, SNSMIL (Shot Noise based Single Molecule Identification and Localization). This algorithm is based on the intrinsic nature of noise, i.e., its Poisson or shot noise characteristics and a new identification criterion, QS

SUBMITTER: Tang Y 

PROVIDER: S-EPMC4476421 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature