Profiling molecular and behavioral circadian rhythms in the non-symbiotic sea anemone Nematostella vectensis.

ABSTRACT: Endogenous circadian clocks are poorly understood within early-diverging animal lineages. We have characterized circadian behavioral patterns and identified potential components of the circadian clock in the starlet sea anemone, Nematostella vectensis: a model cnidarian which lacks algal symbionts. Using automatic video tracking we showed that Nematostella exhibits rhythmic circadian locomotor activity, which is persistent in constant dark, shifted or disrupted by external dark/light cues and maintained the same rate at two different temperatures. This activity was inhibited by a casein kinase 1δ/ε inhibitor, suggesting a role for CK1 homologue(s) in Nematostella clock. Using high-throughput sequencing we profiled Nematostella transcriptomes over 48 hours under a light-dark cycle. We ident

SUBMITTER: Oren M 

PROVIDER: S-EPMC4476465 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature