Mesenchymal stromal cells of osteosarcoma patients do not show evidence of neoplastic changes during long-term culture.

ABSTRACT:

Background

In vitro expanded mesenchymal stromal cells (MSCs) are increasingly used as experimental cellular therapy. However, there have been concerns regarding the safety of their use, particularly with regard to possible oncogenic transformation. MSCs are the hypothesized precursor cells of high-grade osteosarcoma, a tumor with often complex karyotypes occurring mainly in adolescents and young adults.

Methods

To determine if MSCs from osteosarcoma patients could be predisposed to malignant transformation we cultured MSCs of nine osteosarcoma patients and five healthy donors for an average of 649 days (range 601-679 days). Also, we compared MSCs derived from osteosarcoma patients at diagnosis and from healthy donors using genome wide gene expression profiling.

Results</

SUBMITTER: Buddingh EP 

PROVIDER: S-EPMC4477606 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature