Sequence-controlled RNA self-processing: computational design, biochemical analysis, and visualization by AFM.

ABSTRACT: Reversible chemistry allowing for assembly and disassembly of molecular entities is important for biological self-organization. Thus, ribozymes that support both cleavage and formation of phosphodiester bonds may have contributed to the emergence of functional diversity and increasing complexity of regulatory RNAs in early life. We have previously engineered a variant of the hairpin ribozyme that shows how ribozymes may have circularized or extended their own length by forming concatemers. Using the Vienna RNA package, we now optimized this hairpin ribozyme variant and selected four different RNA sequences that were expected to circularize more efficiently or form longer concatemers upon transcription. (Two-dimensional) PAGE analysis confirms that (i) all four selected ribozymes are cataly

SUBMITTER: Petkovic S 

PROVIDER: S-EPMC4478344 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature