Diversity of gut microflora is required for the generation of B cell with regulatory properties in a skin graft model.

ABSTRACT: B cells have been reported to promote graft rejection through alloantibody production. However, there is growing evidence that B cells can contribute to the maintenance of tolerance. Here, we used a mouse model of MHC-class I mismatched skin transplantation to investigate the contribution of B cells to graft survival. We demonstrate that adoptive transfer of B cells prolongs skin graft survival but only when the B cells were isolated from mice housed in low sterility "conventional" (CV) facilities and not from mice housed in pathogen free facilities (SPF). However, prolongation of skin graft survival was lost when B cells were isolated from IL-10 deficient mice housed in CV facilities. The suppressive function of B cells isolated from mice housed in CV facilities correlated with an anti-in

SUBMITTER: Alhabbab R 

PROVIDER: S-EPMC4479822 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature