Universal Approach to FRAP Analysis of Arbitrary Bleaching Patterns.

ABSTRACT: The original approach to calculating diffusion coefficients of a fluorescent probe from Fluorescence Recovery After Photobleaching (FRAP) measurements assumes bleaching with a circular laser beam of a Gaussian intensity profile. This method was used without imaging the bleached cell. An empirical equation for calculating diffusion coefficients from a rectangular bleaching geometry, created in a confocal image, was later published, however a single method allowing the calculation of diffusion coefficients for arbitrary geometry does not exist. Our simulation approach allows computation of diffusion coefficients regardless of bleaching geometry used in the FRAP experiment. It accepts a multiple-frame TIFF file, representing the experiment as input, and simulates the (pure) diffusion of the f

SUBMITTER: Blumenthal D 

PROVIDER: S-EPMC4479983 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature