Aging results in differential regulation of DNA repair pathways in pachytene spermatocytes in the Brown Norway rat.

ABSTRACT: The present trend of increasing paternal age is accompanied by concerns for the development of complex multigene diseases (e.g., autism and schizophrenia) in progeny. Recent studies have established strong correlations between male age, increased oxidative stress, decreased sperm quality, and structural aberrations of chromatin and DNA in spermatozoa. We tested the hypothesis that increasing age would result in altered gene expression relating to oxidative stress and DNA damage/repair in germ cells. To test this hypothesis, pachytene spermatocytes and round spermatids were isolated from Brown Norway (BN) rats at 4 (young) and 18 (aged) mo of age. Microarray analysis was used to compare gene expression between the groups. The probe sets with significantly altered expression were linked to D

SUBMITTER: Paul C 

PROVIDER: S-EPMC4480431 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature