Simultaneous recording of fluorescence and electrical signals by photometric patch electrode in deep brain regions in vivo.

ABSTRACT: Despite its widespread use, high-resolution imaging with multiphoton microscopy to record neuronal signals in vivo is limited to the surface of brain tissue because of limited light penetration. Moreover, most imaging studies do not simultaneously record electrical neural activity, which is, however, crucial to understanding brain function. Accordingly, we developed a photometric patch electrode (PME) to overcome the depth limitation of optical measurements and also enable the simultaneous recording of neural electrical responses in deep brain regions. The PME recoding system uses a patch electrode to excite a fluorescent dye and to measure the fluorescence signal as a light guide, to record electrical signal, and to apply chemicals to the recorded cells locally. The optical signal was ana

SUBMITTER: Hirai Y 

PROVIDER: S-EPMC4480622 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature