Screening of hepatocyte proteins binding to F protein of hepatitis C virus by yeast two-hybrid system.

ABSTRACT:

Aim

To investigate the biological function of F protein by yeast two-hybrid system.

Methods

We constructed F protein bait plasmid by cloning the gene of F protein into pGBKT7, then recombinant plasmid DNA was transformed into yeast AH109 (a type). The transformed yeast AH109 was mated with yeast Y187 (alpha type) containing liver cDNA library plasmid in 2XYPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-alpha-gal for selection and screening. After extracting and sequencing plasmids from positive (blue) colonies, we underwent sequence analysis by bioinformatics.

Results

Thirty-six colonies were selected and sequenced. Among them, 11 colonies were zymogen granule protein, 5 colonies were zinc finger protein, 4

SUBMITTER: Huang YP 

PROVIDER: S-EPMC4481484 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature