Evaluation of Appropriate Reference Genes for Gene Expression Normalization during Watermelon Fruit Development.

ABSTRACT: Gene expression analysis in watermelon (Citrullus lanatus) fruit has drawn considerable attention with the availability of genome sequences to understand the regulatory mechanism of fruit development and to improve its quality. Real-time quantitative reverse-transcription PCR (qRT-PCR) is a routine technique for gene expression analysis. However, appropriate reference genes for transcript normalization in watermelon fruits have not been well characterized. The aim of this study was to evaluate the appropriateness of 12 genes for their potential use as reference genes in watermelon fruits. Expression variations of these genes were measured in 48 samples obtained from 12 successive developmental stages of parthenocarpic and fertilized fruits of two watermelon genotypes by using qRT-PCR analy

SUBMITTER: Kong Q 

PROVIDER: S-EPMC4481515 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature