Population-scale three-dimensional reconstruction and quantitative profiling of microglia arbors.

ABSTRACT:

Motivation

The arbor morphologies of brain microglia are important indicators of cell activation. This article fills the need for accurate, robust, adaptive and scalable methods for reconstructing 3-D microglial arbors and quantitatively mapping microglia activation states over extended brain tissue regions.

Results

Thick rat brain sections (100-300 µm) were multiplex immunolabeled for IBA1 and Hoechst, and imaged by step-and-image confocal microscopy with automated 3-D image mosaicing, producing seamless images of extended brain regions (e.g. 5903 × 9874 × 229 voxels). An over-complete dictionary-based model was learned for the image-specific local structure of microglial processes. The microglial arbors were reconstructed seamlessly using an automated and scalable algorith

SUBMITTER: Megjhani M 

PROVIDER: S-EPMC4481841 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature