MultiSNV: a probabilistic approach for improving detection of somatic point mutations from multiple related tumour samples.

ABSTRACT: Somatic variant analysis of a tumour sample and its matched normal has been widely used in cancer research to distinguish germline polymorphisms from somatic mutations. However, due to the extensive intratumour heterogeneity of cancer, sequencing data from a single tumour sample may greatly underestimate the overall mutational landscape. In recent studies, multiple spatially or temporally separated tumour samples from the same patient were sequenced to identify the regional distribution of somatic mutations and study intratumour heterogeneity. There are a number of tools to perform somatic variant calling from matched tumour-normal next-generation sequencing (NGS) data; however none of these allow joint analysis of multiple same-patient samples. We discuss the benefits and challenges of mu

SUBMITTER: Josephidou M 

PROVIDER: S-EPMC4482059 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature