Anti-obesity and metabolic efficacy of the β3-adrenergic agonist, CL316243, in mice at thermoneutrality compared to 22°C.

ABSTRACT:

Objective

Mice are typically housed at environmental temperatures below thermoneutrality, whereas humans live near thermoneutrality. This difference affects energy physiology and, potentially, anti-obesity drug efficacy. Here β3-adrenergic agonist treatment at thermoneutrality (30°C) versus room temperature (22°C) is compared.

Methods

Male C57BL/6J mice were singly housed at 30°C or 22°C and treated with vehicle or CL316243, a β3-agonist, for 4 weeks. Food intake, energy expenditure, body and adipose weight, brown adipose activity, white adipose browning, and glucose tolerance were evaluated. CL316243 treatment was studied in both chow- and high-fat diet-fed mice.

Results

Mice at 30°C, compared to 22°C, had reduced food intake, metabolic rate, and brown adipose activi

SUBMITTER: Xiao C 

PROVIDER: S-EPMC4482793 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature