Wnt pathway in atypical teratoid rhabdoid tumors.

ABSTRACT:

Background

Atypical teratoid rhabdoid tumor (ATRT) is an aggressive pediatric brain tumor with limited therapeutic options. The hypothesis for this study was that the Wnt pathway triggered by the Wnt5B ligand plays an important role in ATRT biology. To address this hypothesis, the role of WNT5B and other Wnt pathway genes was analyzed in ATRT tissues and ATRT primary cell lines.

Methods

Transcriptome-sequencing analyses were performed using nanoString platforms, immunohistochemistry, Western blotting, quantitative reverse transcriptase PCR, immunoprecipitation, short interference RNA studies, cell viability studies, and drug dose response (DDR) assays.

Results

Our transcriptome-sequencing results of Wnt pathway genes from ATRT tissues and cell lines indicated that the

SUBMITTER: Chakravadhanula M 

PROVIDER: S-EPMC4483069 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature