VAMP8 facilitates cellular proliferation and temozolomide resistance in human glioma cells.

ABSTRACT:

Background

Malignant glioma is a common and lethal primary brain tumor in adults. Here we identified a novel oncoprotein, vesicle-associated membrane protein 8 (VAMP8), and investigated its roles in tumorigenisis and chemoresistance in glioma.

Methods

The expression of gene and protein were determined by quantitative PCR and Western blot, respectively. Histological analysis of 282 glioma samples and 12 normal controls was performed by Pearson's chi-squared test. Survival analysis was performed using the log-rank test and Cox proportional hazards regression. Cell proliferation and cytotoxicity assay were conducted using Cell Counting Kit-8. Autophagy was detected by confocal microscopy and Western blot.

Results

VAMP8 was significantly overexpressed in human glioma speci

SUBMITTER: Chen Y 

PROVIDER: S-EPMC4483099 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature