Distribution and gene mutation of enteric flora carrying β-glucuronidase among patients with colorectal cancer.

ABSTRACT:

Objective

To explore the difference of distribution in intestinal flora among colorectal cancer patients and healthy controls and investigate characteristics and changes of sequences in beta-glucuronidase (β-glucuronidase, β-G).

Methods

Bacterial genomic DNA and E. coli DNA in feces were extracted from colorectal cancer patients and healthy controls respectively. Specific primers for β-G gene were designed and amplified by PCR as templates of fecal bacteria genomic DNA and E. coli DNA respectively.

Results

Compared with normal control, the amount of E. coli in cancer group increased significantly, Lactobacillus and Bifidobacterium probiotics reduced significantly, and proportional quantity of anaerobic bacteria and aerobic bacteria reversed. The intestinal flora carry

SUBMITTER: Li Y 

PROVIDER: S-EPMC4483899 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature