Core promoter sequence in yeast is a major determinant of expression level.

ABSTRACT: The core promoter is the regulatory sequence to which RNA polymerase is recruited and where it acts to initiate transcription. Here, we present the first comprehensive study of yeast core promoters, providing massively parallel measurements of core promoter activity and of TSS locations and relative usage for thousands of native and designed sequences. We found core promoter activity to be highly correlated to the activity of the entire promoter and that sequence variation in different core promoter regions substantially tunes its activity in a predictable way. We also show that location, orientation, and flanking bases critically affect TATA element function, that transcription initiation in highly active core promoters is focused within a narrow region, that poly(dA:dT) orientation has a

SUBMITTER: Lubliner S 

PROVIDER: S-EPMC4484384 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature