Enzymatic measurement of phosphatidylglycerol and cardiolipin in cultured cells and mitochondria.

ABSTRACT: Phosphatidylglycerol (PG) and cardiolipin (CL) are synthesized in mitochondria and regulate numerous biological functions. In this study, a novel fluorometric method was developed for measuring PG and CL using combinations of specific enzymes and Amplex Red. This assay quantified the sum of PG and CL (PG + CL) regardless of the species of fatty acyl chain. The calibration curve for PG + CL measurement was linear, and the detection limit was 1 μM (10 pmol in the reaction mixture). This new method was applied to the determinations of PG + CL content in HEK293 cells and CL content in purified mitochondria, because the mitochondrial content of PG is negligible compared with that of CL. We demonstrated that the PG+CL content was greater at low cell density than at high cell density. The overexp

SUBMITTER: Morita SY 

PROVIDER: S-EPMC4485230 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature