Molecular epidemiology and phylogenetic analysis of Dengue virus type-1 and 2 isolated in Malaysia.

ABSTRACT:

Objective

Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus.

Methods

Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree.

Results

A total of 53 dengue virus isolates were positive, of which 38 (71.7%) were DENV-1 and 15 (28.3%) were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. T

SUBMITTER: Chew MH 

PROVIDER: S-EPMC4485282 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature