Ontology highlight
ABSTRACT:
SUBMITTER: Xiao S
PROVIDER: S-EPMC4485594 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Biotechnology and bioengineering 20150616 8
Obtaining adequate quantities of functional mammalian membrane proteins has been a bottleneck in their structural and functional studies because the expression of these proteins from mammalian cells is relatively low. To explore the possibility of enhancing expression of these proteins using miRNA, a stable T-REx-293 cell line expressing the neurotensin receptor type 1 (NTSR1), a hard-to-express G protein-coupled receptor (GPCR), was constructed. The cell line was then subjected to human miRNA m ...[more]