CD4⁺ T cells potently induce epithelial-mesenchymal-transition in premalignant and malignant pancreatic ductal epithelial cells-novel implications of CD4⁺ T cells in pancreatic cancer development.

ABSTRACT: Chronic pancreatitis (CP) is a risk factor of pancreatic ductal adenocarcinoma (PDAC) and characterized by a pronounced desmoplastic reaction with CD4⁺ T cells accounting for the majority of the stromal T cell infiltrate. Epithelial-mesenchymal-transition (EMT) is a critical process for metastasis by which epithelial/carcinoma cells become enabled to disseminate probably prior to tumor formation. To investigate whether CD4⁺ T cells induce EMT in human pancreatic ductal epithelial cells, premalignant H6c7 cells were mono- or co-cultured with human CD4⁺CD25⁺CD127^-CD49d^- regulatory T cells (T-regs) or CD4⁺CD25^- T-effector cells (T-effs) being isolated by negative magnetic bead separation from blood of heal

SUBMITTER: Goebel L 

PROVIDER: S-EPMC4485733 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature