Extracellular vesicle-mediated transfer of functional RNA in the tumor microenvironment.

ABSTRACT: Extracellular vesicles (EVs) have been shown to transfer various molecules, including functional RNA between cells and this process has been suggested to be particularly relevant in tumor-host interactions. However, data on EV-mediated RNA transfer has been obtained primarily by in vitro experiments or involving ex vivo manipulations likely affecting its biology, leaving their physiological relevance unclear. We engineered glioma and carcinoma tumor cells to express Cre recombinase showing their release of EVs containing Cre mRNA in various EV subfractions including exosomes. Transplantation of these genetically modified tumor cells into mice with a Cre reporter background leads to frequent recombination events at the tumor site. In both tumor models the majority of recombine

SUBMITTER: Ridder K 

PROVIDER: S-EPMC4485784 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature