Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation.

ABSTRACT:

Background

Nuclear myosin 1c (NM1) is emerging as a regulator of transcription and chromatin organization.

Results

Using chromatin immunoprecipitation and deep sequencing (ChIP-Seq) in combination with molecular analyses, we investigated the global association of NM1 with the mammalian genome. Analysis of the ChIP-Seq data demonstrates that NM1 binds across the entire mammalian genome with occupancy peaks correlating with distributions of RNA Polymerase II (Pol II) and active epigenetic marks at class II gene promoters. In mouse embryonic fibroblasts subjected to RNAi mediated NM1 gene silencing, we show that NM1 synergizes with polymerase-associated actin to maintain active Pol II at the promoter. NM1 also co-localizes with the nucleosome remodeler SNF2h at class II promote

SUBMITTER: Almuzzaini B 

PROVIDER: S-EPMC4486089 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature