The miRNA Profile of Platelets Stored in a Blood Bank and Its Relation to Cellular Damage from Storage.

ABSTRACT: Millions of blood products are transfused each year, and many lives are directly affected by transfusion. Platelet concentrate (PC) is one of the main products derived from blood. Even under good storage conditions, PC is likely to suffer cell damage. The shape of platelets changes after 5 to 7 days of storage at 22°C. Taking into consideration that some platelet proteins undergo changes in their shape and functionality during PC storage. Sixteen PC bags were collected and each PC bag tube was cut into six equal pieces to perform experiments with platelets from six different days of storage. Thus, on the first day of storage, 1/6 of the tube was used for miRNA extraction, and the remaining 5/6 was stored under the same conditions until extraction of miRNAs on each the following five days.

SUBMITTER: Pontes TB 

PROVIDER: S-EPMC4486185 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature