Integrated approaches to miRNAs target definition: time-series analysis in an osteosarcoma differentiative model.

ABSTRACT:

Background

microRNAs (miRs) are small non-coding RNAs involved in the fine regulation of several cellular processes by inhibiting their target genes at post-transcriptional level. Osteosarcoma (OS) is a tumor thought to be related to a molecular blockade of the normal process of osteoblast differentiation. The current paper explores temporal transcriptional modifications comparing an osteosarcoma cell line, Saos-2, and clones stably transfected with CD99, a molecule which was found to drive OS cells to terminally differentiate.

Methods

Parental cell line and CD99 transfectants were cultured up to 14 days in differentiating medium. In this setting, OS cells were profiled by gene and miRNA expression arrays. Integration of gene and miRNA profiling was performed by both sequenc

SUBMITTER: Grilli A 

PROVIDER: S-EPMC4486310 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature