An Automated Homogeneous Immunoassay for Quantitating Imatinib Concentrations in Plasma.

ABSTRACT:

Background

Imatinib pharmacokinetic variability and the relationship of trough concentrations with clinical outcomes have been extensively reported. Although physical methods to quantitate imatinib exist, they are not widely available for routine use. An automated homogenous immunoassay for imatinib has been developed, facilitating routine imatinib testing.

Methods

Imatinib-selective monoclonal antibodies, without substantial cross-reactivity to the N-desmethyl metabolite or N-desmethyl conjugates, were produced. The antibodies were conjugated to 200 nm particles to develop immunoassay reagents on the Beckman Coulter AU480 analyzer. These reagents were analytically validated using Clinical Laboratory Standards Institute protocols. Method comparison to liquid chromatography t

SUBMITTER: Beumer JH 

PROVIDER: S-EPMC4486633 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature