Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds.

ABSTRACT:

Background

Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 (cisΔ9, 12, 15); ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications. The recently identified enzyme: phosphatidylcholine diacylglycerol cholinephosphotransferase (PDCT), catalyzes the interconversion between phosphatidylcholine (PC) and diacylglycerol (DAG), and has been shown to play an important role in PUFA accumulation in Arabidopsis thaliana seeds.

Methods

Two flax PDCT genes were identified using homology-based approach.

Results

In this study, we describe the isolation and characterization of two PDCT genes from flax (LuPDCT1 and LuPDCT

SUBMITTER: Wickramarathna AD 

PROVIDER: S-EPMC4486708 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature