Co-culture with endometrial stromal cells enhances the differentiation of human embryonic stem cells into endometrium-like cells.

ABSTRACT: In vitro differentiation of human embryonic stem cells (hESCs) into endometrium-like cells may provide a useful tool for clinical treatment. The aim of the present study was to investigate the differentiation potential of hESCs into endometrium-like cells using three methods, which included induction by feeder cells, co-culture with endometrial stromal cells and induction with embryoid bodies. Following differentiation, the majority of cells positively expressed cytokeratin and epithelial cell adhesion molecule (EPCAM). Factors associated with endometrium cell function, namely the estrogen and progesterone receptors (ER and PR), were also detected. At day 21 following the induction of differentiation, the expression levels of cytokeratin, EPCAM, ER and PR were significantly increase

SUBMITTER: Yu W 

PROVIDER: S-EPMC4486790 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature