Beta-cell regeneration from vimentin+/MafB+ cells after STZ-induced extreme beta-cell ablation.

ABSTRACT: Loss of functional beta-cells is fundamental in both type 1 and type 2 diabetes. In situ beta-cell regeneration therefore has garnered great interest as an approach to diabetes therapy. Here, after elimination of pre-existing beta cells by a single high-dose of streptozotocin (STZ), we demonstrated that a considerable amount of beta-like-cells was generated within 48 hrs. But the newly formed insulin producing cells failed to respond to glucose challenge at this time and diminished afterwards. Insulin treatment to normalize the glucose level protected the neogenic beta-like cells and the islet function was also gradually matured. Strikingly, intermediate cells lacking epithelial marker E-cadherin but expressing mesenchymal cell-specific marker vimentin appeared within 16 hrs following STZ

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC4486952 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature