Characterization of pseudorabies virus transcriptome by Illumina sequencing.

ABSTRACT:

Background

Pseudorabies virus is a widely-studied model organism of the Herpesviridae family, with a compact genome arrangement of 72 known coding sequences. In order to obtain an up-to-date genetic map of the virus, a combination of RNA-sequencing approaches were applied, as recent advancements in high-throughput sequencing methods have provided a wealth of information on novel RNA species and transcript isoforms, revealing additional layers of transcriptome complexity in several viral species.

Results

The total RNA content and polyadenylation landscape of pseudorabies virus were characterized for the first time at high coverage by Illumina high-throughput sequencing of cDNA samples collected during the lytic infectious cycle. As anticipated, nearly all of the viral genome

SUBMITTER: Olah P 

PROVIDER: S-EPMC4487798 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature