Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.

ABSTRACT: The widespread availability of programmable site-specific nucleases now enables targeted gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate zebrafish lines bearing individual mutations in more than 20 genes. We found that mutations in only a small proportion of genes caused defects in embryogenesis. Moreover, mutants for ten different genes failed to recapitulate published Morpholino-induced phenotypes (morphants). The absence of phenotypes in mutant embryos was not likely due to maternal effects or failure to eliminate gene function. Consistently, a comparison of published morphant defects with the Sanger Zebrafish Mutation Project revealed that approximately 80% of morphant phenotypes were not observed in mutant embryos, similar to our mutant

SUBMITTER: Kok FO 

PROVIDER: S-EPMC4487878 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature