Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing.

ABSTRACT:

Background

Small ncRNAs (sncRNAs) offer great hope as biomarkers of disease and response to treatment. This has been highlighted in the context of several medical conditions such as cancer, liver disease, cardiovascular disease, and central nervous system disorders, among many others. Here we assessed several steps involved in the development of an ncRNA biomarker discovery pipeline, ranging from sample preparation to bioinformatic processing of small RNA sequencing data.

Methods

A total of 45 biological samples were included in the present study. All libraries were prepared using the Illumina TruSeq Small RNA protocol and sequenced using the HiSeq2500 or MiSeq Illumina sequencers. Small RNA sequencing data was validated using qRT-PCR. At each stage, we evaluated the pros an

SUBMITTER: Lopez JP 

PROVIDER: S-EPMC4487992 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature