Application of BRET to monitor ligand binding to GPCRs.

ABSTRACT: Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

SUBMITTER: Stoddart LA 

PROVIDER: S-EPMC4488387 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature