Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea.

ABSTRACT:

Background

Microalgae have received considerable interest as a source of biofuel production. The unicellular green alga Pseudochoricystis ellipsoidea (non-validated scientific name) strain Obi appears to be suitable for large-scale cultivation in outdoor open ponds for biodiesel production because it accumulates lipids to more than 30 % of dry cell weight under nitrogen-depleted conditions. It also grows rapidly under acidic conditions at which most protozoan grazers of microalgae may not be tolerant. The lipid productivity of this alga could be improved using genetic engineering techniques; however, genetically modified organisms are the subject of regulation by specific laws. Therefore, the aim of this study was to develop a self-cloning-based positive selection system for the br

SUBMITTER: Kasai Y 

PROVIDER: S-EPMC4489027 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature