RNA-Redesign: a web server for fixed-backbone 3D design of RNA.

ABSTRACT: RNA is rising in importance as a design medium for interrogating fundamental biology and for developing therapeutic and bioengineering applications. While there are several online servers for design of RNA secondary structure, there are no tools available for the rational design of 3D RNA structure. Here we present RNA-Redesign (http://rnaredesign.stanford.edu), an online 3D design tool for RNA. This resource utilizes fixed-backbone design to optimize the sequence identity and nucleobase conformations of an RNA to match a desired backbone, analogous to fundamental tools that underlie rational protein engineering. The resulting sequences suggest thermostabilizing mutations that can be experimentally verified. Further, sequence preferences that differ between natural and computationally desi

SUBMITTER: Yesselman JD 

PROVIDER: S-EPMC4489241 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature