Production of recombinant proteins in Mycobacterium smegmatis for structural and functional studies.

ABSTRACT: Protein production using recombinant DNA technology has a fundamental impact on our understanding of biology through providing proteins for structural and functional studies. Escherichia coli (E. coli) has been traditionally used as the default expression host to over-express and purify proteins from many different organisms. E. coli does, however, have known shortcomings for obtaining soluble, properly folded proteins suitable for downstream studies. These shortcomings are even more pronounced for the mycobacterial pathogen Mycobacterium tuberculosis, the bacterium that causes tuberculosis, with typically only one third of proteins expressed in E. coli produced as soluble proteins. Mycobacterium smegmatis (M. smegmatis) is a closely related and non-pathogenic species that has been success

SUBMITTER: Bashiri G 

PROVIDER: S-EPMC4489920 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature