Laparoscopic Fluorescent Visualization of the Ureter With Intravenous IRDye800CW.

ABSTRACT:

Objectives

Ureter injury is a serious complication of laparoscopic surgery. Current strategies to identify the ureters, such as placement of a ureteral stent, carry additional risks for patients. We hypothesize that the systemically injected near-infrared (NIR) dye IRDye800CW-CA can be used to visualize ureters intraoperatively.

Methods

Adult female mixed-breed pigs weighing 24 to 41 kg (n = 2 per dose) were given a 30, 60, or 120 μg/kg systemic injection of IRDye800CW-CA. Using the Food and Drug Administration-cleared Pinpoint laparoscopic NIR system, images of the ureter and bladder were captured every 10 minutes for 60 minutes after injection. To determine the biodistribution of the dye, tissues were collected for ex vivo analysis with the Pearl Impulse system. ImageJ sof

SUBMITTER: Korb ML 

PROVIDER: S-EPMC4490072 | biostudies-literature | 2015 Jul-Aug

REPOSITORIES: biostudies-literature