Functional analysis of microRNA activity in Brugia malayi.

ABSTRACT: The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.

SUBMITTER: Liu C 

PROVIDER: S-EPMC4490087 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature