Stro-1/CD44 as putative human myometrial and fibroid stem cell markers.

ABSTRACT:

Objective

To identify and characterize myometrial/fibroid stem cells by specific stem cell markers in human myometrium, and to better understand the stem cell contribution in the development of uterine fibroids.

Design

Prospective, experimental human and animal study.

Setting

University research laboratory.

Patient(s)/animal(s)

Women undergoing hysterectomy for treatment of symptomatic uterine fibroids and female NOD/SCID/IL-2Rγ(null) mice.

Intervention(s)

Identification and isolation of stem cells from human fibroids and adjacent myometrium tissues using Stro-1/CD44-specific surface markers.

Main outcome measure(s)

Flow cytometry, semiquantitative polymerase chain reaction, clonogenicity assays, cell culture, molecular analysis, immunocyto-histoc

SUBMITTER: Mas A 

PROVIDER: S-EPMC4490118 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature