In vivo bioimaging with tissue-specific transcription factor activated luciferase reporters.

ABSTRACT: The application of transcription factor activated luciferase reporter cassettes in vitro is widespread but potential for in vivo application has not yet been realized. Bioluminescence imaging enables non-invasive tracking of gene expression in transfected tissues of living rodents. However the mature immune response limits luciferase expression when delivered in adulthood. We present a novel approach of tissue-targeted delivery of transcription factor activated luciferase reporter lentiviruses to neonatal rodents as an alternative to the existing technology of generating germline transgenic light producing rodents. At this age, neonates acquire immune tolerance to the conditionally responsive luciferase reporter. This simple and transferrable procedure permits surrogate quantitation of tra

SUBMITTER: Buckley SM 

PROVIDER: S-EPMC4490336 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature