The temporal and spatial expression patterns of ABCG2 in the developing human heart.

ABSTRACT:

Background

The discovery that the adult heart is not a terminally differentiated organ and contains stem/progenitor cells has important implications for the development of cellular therapeutics to treat heart disease. Moreover the discovery of cardiac stem cells might be important in furthering our understanding of both normal and abnormal cardiac development and yet little is known about these cell populations in the developing human heart, which we have focused on in this study.

Methods

The presence of ABCG2 and islet-1 expressing cells in human heart was determined using immunohistochemistry and RT-PCR (and western blotting for ABCG2). Cardiac SP cells were isolated using FACS. Co-localisation immunohistochemistry was used to determine if ABCG2 positive cells expressed ot

SUBMITTER: Alfakir M 

PROVIDER: S-EPMC4490625 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature