Microarray profiling of long non-coding RNA (lncRNA) associated with hypertrophic cardiomyopathy.

ABSTRACT:

Background

Hypertrophic cardiomyopathy (HCM) is an inherited disorder with around 1400 known mutations; however the molecular pathways leading from genotype to phenotype are not fully understood. LncRNAs, which account for approximately 98 % of human genome, are becoming increasingly interesting with regard to various diseases. However, changes in the expression of regulatory lncRNAs in HCM have not yet been reported. To identify myocardial lncRNAs involved in HCM and characterize their roles in HCM pathogenesis.

Methods

Myocardial tissues were obtained from 7 HCM patients and 5 healthy individuals, and lncRNA and mRNA expression profiles were analyzed using the Arraystar human lncRNA microarray. Real-time PCR was conducted to validate the expression pattern of lncRNA and mR

SUBMITTER: Yang W 

PROVIDER: S-EPMC4490660 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature