Laser system refinements to reduce variability in infarct size in the rat photothrombotic stroke model.

ABSTRACT:

Background

The rat photothrombotic stroke model can induce brain infarcts with reasonable biological variability. Nevertheless, we observed unexplained high inter-individual variability despite using a rigorous protocol. Of the three major determinants of infarct volume, photosensitive dye concentration and illumination period were strictly controlled, whereas undetected fluctuation in laser power output was suspected to account for the variability.

New method

The frequently utilized Diode Pumped Solid State (DPSS) lasers emitting 532 nm (green) light can exhibit fluctuations in output power due to temperature and input power alterations. The polarization properties of the Nd:YAG and Nd:YVO4 crystals commonly used in these lasers are another potential source of fluctuation,

SUBMITTER: Alaverdashvili M 

PROVIDER: S-EPMC4490890 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature