Cholesterol regulates multiple forms of vesicle endocytosis at a mammalian central synapse.

ABSTRACT: Endocytosis in synapses sustains neurotransmission by recycling vesicle membrane and maintaining the homeostasis of synaptic membrane. A role of membrane cholesterol in synaptic endocytosis remains controversial because of conflicting observations, technical limitations in previous studies, and potential interference from non-specific effects after cholesterol manipulation. Furthermore, it remains unclear whether cholesterol participates in distinct forms of endocytosis that function under different activity levels. In this study, applying the whole-cell membrane capacitance measurement to monitor endocytosis in real time at the rat calyx of Held terminals, we found that disrupting cholesterol with dialysis of cholesterol oxidase or methyl-β-cyclodextrin impaired three different forms of e

SUBMITTER: Yue HY 

PROVIDER: S-EPMC4490978 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature