PLCζ disruption with complete fertilization failure in normozoospermia.

ABSTRACT:

Purpose

Intracytoplasmic sperm injection (ICSI) is widely used to achieve fertilization in the presence of severe male factor, resulting in high fertilization rates. Nevertheless, 1-3 % of couples experience complete fertilization failure after ICSI. When a male factor is identified, assisted oocyte activation (AOA) can help overcome fertilization failures. The objective of this study is to describe a case of repeated complete fertilization failures after ICSI with donor oocytes, and to investigate the molecular and functional aspects of phospholipase C zeta (PLCζ) protein in the patient semen.

Methods

The patient was a normozoospermic male who had previously fathered, through natural conception, four children by a different partner. Molecular and functional analysis of sper

SUBMITTER: Durban M 

PROVIDER: S-EPMC4491077 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature