Structural studies of P-type ATPase-ligand complexes using an X-ray free-electron laser.

ABSTRACT: Membrane proteins are key players in biological systems, mediating signalling events and the specific transport of e.g. ions and metabolites. Consequently, membrane proteins are targeted by a large number of currently approved drugs. Understanding their functions and molecular mechanisms is greatly dependent on structural information, not least on complexes with functionally or medically important ligands. Structure determination, however, is hampered by the difficulty of obtaining well diffracting, macroscopic crystals. Here, the feasibility of X-ray free-electron-laser-based serial femtosecond crystallography (SFX) for the structure determination of membrane protein-ligand complexes using microcrystals of various native-source and recombinant P-type ATPase complexes is demonstrated. The

SUBMITTER: Bublitz M 

PROVIDER: S-EPMC4491313 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature